Refubium - Qualitative und quantitative Bestimmung …
For further evaluation of infectivity selectetd PCR positive water specismens werde examined for EntV by cell culture. Hybridization conditions, including 1000 mM LiCl buffer and temperatures of 65°C for three hours, warranted the lysis of enterovirus and a high efficiency of capturing 5'NCR primers to the virale nucleic acid [50].